The impact of biomechanics on corneal endothelium tissue engineering

The integrity of innermost layer the cornea, corneal endothelium, is key to sustaining transparency. Therefore, disease or injury causing loss damage endothelial cell population may threaten vision. Transplantation tissue standard treatment used replace malfunctioning cells. However, this surgery dependent upon donor tissue, which limited in supply. Hence, engineers have attempted construct alternative transplantable tissues therapies alleviate problem. Nevertheless, intrinsic non-dividing nature cells continues foil scientists their attempts yield large numbers laboratory for use such novel therapies. Interestingly, contribution biomechanical properties underlying extracellular matrix (ECM) on division, development and maintenance has been extensively investigated other many types. impact biomechanics behaviour relatively unexplored. Here, we describe contemporary engineering solutions aimed at circumventing scarcity. We review ECM structure features discuss alterations progression point out role developing a tissue-engineered endothelium. highlight main cues, including topographical mechanical features, that cellular behaviors. Finally, influence cues development, how response individual stimuli engineering, implications designing an engineered endothelium maintaining function.